Construct: shRNA TRCN0000232284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020051.1-460s21c1
DNA Barcode:
CTCAGAGCTGCCATCAAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ascl3 (56787)
Gene Description:
achaete-scute family bHLH transcription factor 3
Transcript:
RefSeq NM_020051.1 (CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGAGCTGCCATCAAATAT
Hairpin Sequence:
5'-CCGG-CTCAGAGCTGCCATCAAATAT-CTCGAG-ATATTTGATGGCAGCTCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGAGCTGCCATCAAATATCTCGAGATATTTGATGGCAGCTCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGAGCTGCCATCAAATATCTCGAGATATTTGATGGCAGCTCTGAG-3'

Other clones with same target sequence:

(none)