Construct: shRNA TRCN0000234207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020028.3-330s21c1
DNA Barcode:
ACGCTTCCACCAGCCCATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lpar2 (53978)
Gene Description:
lysophosphatidic acid receptor 2
Transcript:
RefSeq NM_020028.3 (CURRENT)
Match location:
Position 330 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCTTCCACCAGCCCATATA
Hairpin Sequence:
5'-CCGG-ACGCTTCCACCAGCCCATATA-CTCGAG-TATATGGGCTGGTGGAAGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCTTCCACCAGCCCATATACTCGAGTATATGGGCTGGTGGAAGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCTTCCACCAGCCCATATACTCGAGTATATGGGCTGGTGGAAGCGT-3'

Other clones with same target sequence:

(none)