Construct: shRNA TRCN0000235814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010813.3-1016s21c1
DNA Barcode:
CACCGGCTGAAGAAGCCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mnt (17428)
Gene Description:
max binding protein
Transcript:
RefSeq NM_010813.3 (CURRENT)
Match location:
Position 1016 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCGGCTGAAGAAGCCAAAT
Hairpin Sequence:
5'-CCGG-CACCGGCTGAAGAAGCCAAAT-CTCGAG-ATTTGGCTTCTTCAGCCGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCGGCTGAAGAAGCCAAATCTCGAGATTTGGCTTCTTCAGCCGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCGGCTGAAGAAGCCAAATCTCGAGATTTGGCTTCTTCAGCCGGTG-3'

Other clones with same target sequence:

(none)