Construct: shRNA TRCN0000235831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009029.2-3980s21c1
DNA Barcode:
TAAGCCAATCTGGTCTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rb1 (19645)
Gene Description:
RB transcriptional corepressor 1
Transcript:
RefSeq NM_009029.2 (CURRENT)
Match location:
Position 3980 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGCCAATCTGGTCTAATAA
Hairpin Sequence:
5'-CCGG-TAAGCCAATCTGGTCTAATAA-CTCGAG-TTATTAGACCAGATTGGCTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGCCAATCTGGTCTAATAACTCGAGTTATTAGACCAGATTGGCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGCCAATCTGGTCTAATAACTCGAGTTATTAGACCAGATTGGCTTA-3'

Other clones with same target sequence:

(none)