Construct: shRNA TRCN0000235952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027934.2-591s21c1
DNA Barcode:
TAATGAGACCAGCGCTGAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf180 (71816)
Gene Description:
ring finger protein 180
Transcript:
RefSeq NM_027934.2 (CURRENT)
Match location:
Position 591 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATGAGACCAGCGCTGAAAC
Hairpin Sequence:
5'-CCGG-TAATGAGACCAGCGCTGAAAC-CTCGAG-GTTTCAGCGCTGGTCTCATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATGAGACCAGCGCTGAAACCTCGAGGTTTCAGCGCTGGTCTCATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATGAGACCAGCGCTGAAACCTCGAGGTTTCAGCGCTGGTCTCATTA-3'

Other clones with same target sequence:

(none)