Construct: shRNA TRCN0000235979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016781.2-1320s21c1
DNA Barcode:
TTGTGCTCCTCGGGTAGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkag1 (19082)
Gene Description:
protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Transcript:
RefSeq NM_016781.2 (CURRENT)
Match location:
Position 1320 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTGCTCCTCGGGTAGATTC
Hairpin Sequence:
5'-CCGG-TTGTGCTCCTCGGGTAGATTC-CTCGAG-GAATCTACCCGAGGAGCACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTGCTCCTCGGGTAGATTCCTCGAGGAATCTACCCGAGGAGCACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTGCTCCTCGGGTAGATTCCTCGAGGAATCTACCCGAGGAGCACAA-3'

Other clones with same target sequence:

(none)