Construct: shRNA TRCN0000238216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023835.2-1285s21c1
DNA Barcode:
ACTCTAGGCCACCAATAGATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim12a (76681)
Gene Description:
tripartite motif-containing 12A
Transcript:
RefSeq NM_023835.2 (CURRENT)
Match location:
Position 1285 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCTAGGCCACCAATAGATG
Hairpin Sequence:
5'-CCGG-ACTCTAGGCCACCAATAGATG-CTCGAG-CATCTATTGGTGGCCTAGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCTAGGCCACCAATAGATGCTCGAGCATCTATTGGTGGCCTAGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCTAGGCCACCAATAGATGCTCGAGCATCTATTGGTGGCCTAGAGT-3'

Other clones with same target sequence:

(none)