Construct: shRNA TRCN0000238217

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023835.2-1015s21c1
DNA Barcode:
ACAGAGGCTCATCGCTACTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim12a (76681)
Gene Description:
tripartite motif-containing 12A
Transcript:
RefSeq NM_023835.2 (CURRENT)
Match location:
Position 1015 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238217)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGAGGCTCATCGCTACTAG
Hairpin Sequence:
5'-CCGG-ACAGAGGCTCATCGCTACTAG-CTCGAG-CTAGTAGCGATGAGCCTCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGAGGCTCATCGCTACTAGCTCGAGCTAGTAGCGATGAGCCTCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGAGGCTCATCGCTACTAGCTCGAGCTAGTAGCGATGAGCCTCTGT-3'

Other clones with same target sequence:

(none)