Construct: shRNA TRCN0000238505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001044308.2-3316s21c1
DNA Barcode:
AGCTATGATCAGCGATCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacna1i (239556)
Gene Description:
calcium channel, voltage-dependent, alpha 1I subunit
Transcript:
RefSeq NM_001044308.2 (CURRENT)
Match location:
Position 3316 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTATGATCAGCGATCTTTG
Hairpin Sequence:
5'-CCGG-AGCTATGATCAGCGATCTTTG-CTCGAG-CAAAGATCGCTGATCATAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTATGATCAGCGATCTTTGCTCGAGCAAAGATCGCTGATCATAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTATGATCAGCGATCTTTGCTCGAGCAAAGATCGCTGATCATAGCT-3'

Other clones with same target sequence:

(none)