Construct: shRNA TRCN0000238651

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001045527.1-769s21c1
DNA Barcode:
CGGTCATTCCGGCGAGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsf5 (327992)
Gene Description:
heat shock transcription factor family member 5
Transcript:
RefSeq NM_001045527.1 (CURRENT)
Match location:
Position 769 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238651)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTCATTCCGGCGAGATAAT
Hairpin Sequence:
5'-CCGG-CGGTCATTCCGGCGAGATAAT-CTCGAG-ATTATCTCGCCGGAATGACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTCATTCCGGCGAGATAATCTCGAGATTATCTCGCCGGAATGACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTCATTCCGGCGAGATAATCTCGAGATTATCTCGCCGGAATGACCG-3'

Other clones with same target sequence:

(none)