Construct: shRNA TRCN0000238691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081298.1-544s21c1
DNA Barcode:
TACCGCACCGATACGTTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrl2 (99633)
Gene Description:
adhesion G protein-coupled receptor L2
Transcript:
RefSeq NM_001081298.1 (CURRENT)
Match location:
Position 544 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCGCACCGATACGTTAATA
Hairpin Sequence:
5'-CCGG-TACCGCACCGATACGTTAATA-CTCGAG-TATTAACGTATCGGTGCGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCGCACCGATACGTTAATACTCGAGTATTAACGTATCGGTGCGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCGCACCGATACGTTAATACTCGAGTATTAACGTATCGGTGCGGTA-3'

Other clones with same target sequence:

(none)