Construct: shRNA TRCN0000238976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027195.2-616s21c1
DNA Barcode:
TGATCGAGAAATGGGTCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Casz1 (69743)
Gene Description:
castor zinc finger 1
Transcript:
RefSeq NM_027195.2 (CURRENT)
Match location:
Position 616 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000238976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCGAGAAATGGGTCAATG
Hairpin Sequence:
5'-CCGG-TGATCGAGAAATGGGTCAATG-CTCGAG-CATTGACCCATTTCTCGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCGAGAAATGGGTCAATGCTCGAGCATTGACCCATTTCTCGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCGAGAAATGGGTCAATGCTCGAGCATTGACCCATTTCTCGATCA-3'

Other clones with same target sequence:

(none)