Construct: shRNA TRCN0000240559

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007789.3-3132s21c1
DNA Barcode:
TATGCAGCCCTTGCGAGAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncan (13004)
Gene Description:
neurocan
Transcript:
RefSeq NM_007789.3 (CURRENT)
Match location:
Position 3132 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240559)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGCAGCCCTTGCGAGAATG
Hairpin Sequence:
5'-CCGG-TATGCAGCCCTTGCGAGAATG-CTCGAG-CATTCTCGCAAGGGCTGCATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGCAGCCCTTGCGAGAATGCTCGAGCATTCTCGCAAGGGCTGCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGCAGCCCTTGCGAGAATGCTCGAGCATTCTCGCAAGGGCTGCATA-3'

Other clones with same target sequence:

(none)