Construct: shRNA TRCN0000240645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011273.2-526s21c1
DNA Barcode:
ACATTGCGACAGCGCAGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xpr1 (19775)
Gene Description:
xenotropic and polytropic retrovirus receptor 1
Transcript:
RefSeq NM_011273.2 (CURRENT)
Match location:
Position 526 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATTGCGACAGCGCAGAAAG
Hairpin Sequence:
5'-CCGG-ACATTGCGACAGCGCAGAAAG-CTCGAG-CTTTCTGCGCTGTCGCAATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATTGCGACAGCGCAGAAAGCTCGAGCTTTCTGCGCTGTCGCAATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATTGCGACAGCGCAGAAAGCTCGAGCTTTCTGCGCTGTCGCAATGT-3'

Other clones with same target sequence:

(none)