Construct: shRNA TRCN0000240752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013745.5-406s21c1
DNA Barcode:
TCCACGTACCACACGTCTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nufip1 (27275)
Gene Description:
nuclear fragile X mental retardation protein interacting protein 1
Transcript:
RefSeq NM_013745.5 (CURRENT)
Match location:
Position 406 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCACGTACCACACGTCTTAC
Hairpin Sequence:
5'-CCGG-TCCACGTACCACACGTCTTAC-CTCGAG-GTAAGACGTGTGGTACGTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCACGTACCACACGTCTTACCTCGAGGTAAGACGTGTGGTACGTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCACGTACCACACGTCTTACCTCGAGGTAAGACGTGTGGTACGTGGA-3'

Other clones with same target sequence:

(none)