Construct: shRNA TRCN0000241153

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026879.2-1158s21c1
DNA Barcode:
GCCATTAGGCGTCAACGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chmp2b (68942)
Gene Description:
charged multivesicular body protein 2B
Transcript:
RefSeq NM_026879.2 (CURRENT)
Match location:
Position 1158 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241153)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTAGGCGTCAACGTAAA
Hairpin Sequence:
5'-CCGG-GCCATTAGGCGTCAACGTAAA-CTCGAG-TTTACGTTGACGCCTAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTAGGCGTCAACGTAAACTCGAGTTTACGTTGACGCCTAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTAGGCGTCAACGTAAACTCGAGTTTACGTTGACGCCTAATGGC-3'

Other clones with same target sequence:

(none)