Construct: shRNA TRCN0000241213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173863.2-3616s21c1
DNA Barcode:
CCTTCTCACACAGTAAGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crtc3 (70461)
Gene Description:
CREB regulated transcription coactivator 3
Transcript:
RefSeq NM_173863.2 (CURRENT)
Match location:
Position 3616 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTCACACAGTAAGTAAT
Hairpin Sequence:
5'-CCGG-CCTTCTCACACAGTAAGTAAT-CTCGAG-ATTACTTACTGTGTGAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTCACACAGTAAGTAATCTCGAGATTACTTACTGTGTGAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTCACACAGTAAGTAATCTCGAGATTACTTACTGTGTGAGAAGG-3'

Other clones with same target sequence:

(none)