Construct: shRNA TRCN0000241246

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001480221.1-605s21c1
DNA Barcode:
GAATTGACTATAGGGCTATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spdya (70891)
Gene Description:
speedy/RINGO cell cycle regulator family, member A
Transcript:
RefSeq XM_001480221.1 (NON-CURRENT)
Match location:
Position 605 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241246)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTGACTATAGGGCTATTG
Hairpin Sequence:
5'-CCGG-GAATTGACTATAGGGCTATTG-CTCGAG-CAATAGCCCTATAGTCAATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTGACTATAGGGCTATTGCTCGAGCAATAGCCCTATAGTCAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTGACTATAGGGCTATTGCTCGAGCAATAGCCCTATAGTCAATTC-3'

Other clones with same target sequence:

  1. TRCN0000416104