Construct: shRNA TRCN0000416104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182756.3-715s21c1
DNA Barcode:
GAATTGACTATAGGGCTATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPDYA (245711)
Gene Description:
speedy/RINGO cell cycle regulator family member A
Transcript:
RefSeq NM_182756.3 (NON-CURRENT)
Match location:
Position 715 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTGACTATAGGGCTATTG
Hairpin Sequence:
5'-CCGG-GAATTGACTATAGGGCTATTG-CTCGAG-CAATAGCCCTATAGTCAATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTGACTATAGGGCTATTGCTCGAGCAATAGCCCTATAGTCAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTGACTATAGGGCTATTGCTCGAGCAATAGCCCTATAGTCAATTC-3'

Other clones with same target sequence:

  1. TRCN0000241246