Construct: shRNA TRCN0000241448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028785.3-2920s21c1
DNA Barcode:
GCAGCCGGATGTCTTACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dock8 (76088)
Gene Description:
dedicator of cytokinesis 8
Transcript:
RefSeq NM_028785.3 (CURRENT)
Match location:
Position 2920 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCGGATGTCTTACTATT
Hairpin Sequence:
5'-CCGG-GCAGCCGGATGTCTTACTATT-CTCGAG-AATAGTAAGACATCCGGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCGGATGTCTTACTATTCTCGAGAATAGTAAGACATCCGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCGGATGTCTTACTATTCTCGAGAATAGTAAGACATCCGGCTGC-3'

Other clones with same target sequence:

(none)