Construct: shRNA TRCN0000241460

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013608.2-4777s21c1
DNA Barcode:
ACGTGCCATTAACCTAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ercc6l2 (76251)
Gene Description:
excision repair cross-complementing rodent repair deficiency, complementation group 6 like 2
Transcript:
RefSeq NM_001013608.2 (CURRENT)
Match location:
Position 4777 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241460)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGCCATTAACCTAGATAT
Hairpin Sequence:
5'-CCGG-ACGTGCCATTAACCTAGATAT-CTCGAG-ATATCTAGGTTAATGGCACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGCCATTAACCTAGATATCTCGAGATATCTAGGTTAATGGCACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGCCATTAACCTAGATATCTCGAGATATCTAGGTTAATGGCACGT-3'

Other clones with same target sequence:

(none)