Construct: shRNA TRCN0000241682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172557.2-729s21c1
DNA Barcode:
CTATGAGCCCGAGGCCTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rufy1 (216724)
Gene Description:
RUN and FYVE domain containing 1
Transcript:
RefSeq NM_172557.2 (CURRENT)
Match location:
Position 729 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGAGCCCGAGGCCTTAAT
Hairpin Sequence:
5'-CCGG-CTATGAGCCCGAGGCCTTAAT-CTCGAG-ATTAAGGCCTCGGGCTCATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGAGCCCGAGGCCTTAATCTCGAGATTAAGGCCTCGGGCTCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGAGCCCGAGGCCTTAATCTCGAGATTAAGGCCTCGGGCTCATAG-3'

Other clones with same target sequence:

(none)