Construct: shRNA TRCN0000242163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081132.1-4018s21c1
DNA Barcode:
CTAGAGAGTTGCGAATCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Upf2 (326622)
Gene Description:
UPF2 regulator of nonsense transcripts homolog (yeast)
Transcript:
RefSeq NM_001081132.1 (CURRENT)
Match location:
Position 4018 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGAGAGTTGCGAATCTAAA
Hairpin Sequence:
5'-CCGG-CTAGAGAGTTGCGAATCTAAA-CTCGAG-TTTAGATTCGCAACTCTCTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGAGAGTTGCGAATCTAAACTCGAGTTTAGATTCGCAACTCTCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGAGAGTTGCGAATCTAAACTCGAGTTTAGATTCGCAACTCTCTAG-3'

Other clones with same target sequence:

(none)