Construct: shRNA TRCN0000242660

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005951.2-142s21c1
DNA Barcode:
GCAAATGCACCTCCTGCAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MT1H (4496)
Gene Description:
metallothionein 1H
Transcript:
RefSeq NM_005951.2 (CURRENT)
Match location:
Position 142 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242660)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAATGCACCTCCTGCAAGA
Hairpin Sequence:
5'-CCGG-GCAAATGCACCTCCTGCAAGA-CTCGAG-TCTTGCAGGAGGTGCATTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAATGCACCTCCTGCAAGACTCGAGTCTTGCAGGAGGTGCATTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAATGCACCTCCTGCAAGACTCGAGTCTTGCAGGAGGTGCATTTGC-3'

Other clones with same target sequence:

(none)