Transcript: Human NM_005951.2

Homo sapiens metallothionein 1H (MT1H), mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
MT1H (4496)
Length:
397
CDS:
72..257

Additional Resources:

NCBI RefSeq record:
NM_005951.2
NBCI Gene record:
MT1H (4496)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005951.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000072611 CAAATGCACCTCCTGCAAGAA pLKO.1 143 CDS 100% 4.950 2.475 Y MT1F n/a
2 TRCN0000155121 CAAATGCACCTCCTGCAAGAA pLKO.1 143 CDS 100% 4.950 2.475 Y MT1X n/a
3 TRCN0000242659 AGAAGAGCTGCTGCTCCTGTT pLKO_005 160 CDS 100% 4.050 2.025 Y MT1H n/a
4 TRCN0000440406 ATGCACCTCCTGCAAGAAGAG pLKO_005 146 CDS 100% 4.050 2.025 Y MT1A n/a
5 TRCN0000242660 GCAAATGCACCTCCTGCAAGA pLKO_005 142 CDS 100% 4.050 2.025 Y MT1H n/a
6 TRCN0000364944 TGCAAATGCACCTCCTGCAAG pLKO_005 141 CDS 100% 4.050 2.025 Y MT1E n/a
7 TRCN0000376452 TGCAGCTGCTGTGCCTGATGT pLKO_005 240 CDS 100% 1.650 0.825 Y MT1E n/a
8 TRCN0000425748 AGTGCAGCTGCTGTGCCTGAT pLKO_005 238 CDS 100% 1.350 0.675 Y MT1X n/a
9 TRCN0000242865 CCTGCAAGAAGAGCTGCTGCT pLKO_005 154 CDS 100% 0.720 0.360 Y MT1G n/a
10 TRCN0000219454 CTGCAAGAAGAGCTGCTGCTC pLKO.1 155 CDS 100% 0.720 0.360 Y Mt1 n/a
11 TRCN0000242658 CCCAGGGCTGCATCTGCAAAG pLKO_005 205 CDS 100% 0.000 0.000 Y MT1H n/a
12 TRCN0000257215 GCCGGCTCCTGCAAGTGCAAA pLKO_005 117 CDS 100% 0.000 0.000 Y MT1H n/a
13 TRCN0000364945 CCTGCAAGAAGAGCTGCTGTT pLKO_005 154 CDS 100% 4.050 2.025 Y MT1E n/a
14 TRCN0000219901 CGGCTCCTGCAAGTGCAAAGA pLKO.1 119 CDS 100% 1.650 0.825 Y MT1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005951.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06599 pDONR223 100% 99.4% 100% None 66A>G n/a
2 ccsbBroad304_06599 pLX_304 0% 99.4% 100% V5 66A>G n/a
3 TRCN0000474764 TTGATAACTTTGGCATCACCATAC pLX_317 100% 99.4% 100% V5 66A>G n/a
4 ccsbBroadEn_06598 pDONR223 100% 92.8% 86.8% None (many diffs) n/a
5 ccsbBroad304_06598 pLX_304 0% 92.8% 86.8% V5 (many diffs) n/a
6 TRCN0000473594 TGCAAGCTAGAGCAGCCGCGTATG pLX_317 100% 92.8% 86.8% V5 (many diffs) n/a
7 ccsbBroadEn_01044 pDONR223 100% 92.8% 88.5% None (many diffs) n/a
8 ccsbBroad304_01044 pLX_304 0% 92.8% 88.5% V5 (many diffs) n/a
9 TRCN0000475214 ATCGACCACCTTCTCGGATCAACG pLX_317 100% 92.8% 88.5% V5 (many diffs) n/a
10 ccsbBroadEn_01040 pDONR223 100% 92.8% 90.1% None (many diffs) n/a
11 ccsbBroad304_01040 pLX_304 0% 92.8% 90.1% V5 (many diffs) n/a
12 TRCN0000465872 GTAGAGTCACTGCCTTGTCCTAGG pLX_317 100% 92.8% 90.1% V5 (many diffs) n/a
13 ccsbBroadEn_01043 pDONR223 100% 92.8% 88.5% None (many diffs) n/a
14 ccsbBroad304_01043 pLX_304 0% 92.8% 88.5% V5 (many diffs) n/a
15 ccsbBroadEn_01045 pDONR223 100% 91.8% 90.1% None (many diffs) n/a
16 ccsbBroad304_01045 pLX_304 0% 91.8% 90.1% V5 (many diffs) n/a
17 TRCN0000472152 TGCCGATATAGCTCTCTATACTCA pLX_317 100% 91.8% 90.1% V5 (many diffs) n/a
18 ccsbBroadEn_01042 pDONR223 100% 91.2% 81.9% None (many diffs) n/a
19 ccsbBroad304_01042 pLX_304 0% 91.2% 81.9% V5 (many diffs) n/a
20 TRCN0000470527 TTTTTAACCATTGCTGGCACAAAG pLX_317 100% 91.2% 81.9% V5 (many diffs) n/a
21 ccsbBroadEn_01041 pDONR223 100% 91.2% 86.8% None (many diffs) n/a
22 ccsbBroad304_01041 pLX_304 95.5% 91.2% 86.8% V5 (many diffs) n/a
23 ccsbBroadEn_01039 pDONR223 100% 89.6% 86.8% None (many diffs) n/a
24 ccsbBroad304_01039 pLX_304 0% 89.6% 86.8% V5 (many diffs) n/a
25 TRCN0000468729 CAAAATATTAAAACTGATCGTATT pLX_317 100% 89.6% 86.8% V5 (many diffs) n/a
26 ccsbBroadEn_13207 pDONR223 100% 88.5% 83.6% None (many diffs) n/a
27 ccsbBroad304_13207 pLX_304 0% 88.5% 83.6% V5 (many diffs) n/a
28 TRCN0000467056 AGATTTTCATGCAACTTTTCTCGA pLX_317 100% 88.5% 83.6% V5 (many diffs) n/a
29 ccsbBroadEn_10353 pDONR223 100% 71.5% 62.2% None (many diffs) n/a
30 ccsbBroad304_10353 pLX_304 0% 71.5% 62.2% V5 (many diffs) n/a
31 TRCN0000473861 CGCCCGCAGATCATTCGATACGCC pLX_317 100% 71.5% 62.2% V5 (many diffs) n/a
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