Construct: shRNA TRCN0000243512

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172739.4-1324s21c1
DNA Barcode:
AGGGCGTTGAGCGGTACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap35 (232906)
Gene Description:
Rho GTPase activating protein 35
Transcript:
RefSeq NM_172739.4 (CURRENT)
Match location:
Position 1324 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243512)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGCGTTGAGCGGTACATTA
Hairpin Sequence:
5'-CCGG-AGGGCGTTGAGCGGTACATTA-CTCGAG-TAATGTACCGCTCAACGCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGCGTTGAGCGGTACATTACTCGAGTAATGTACCGCTCAACGCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGCGTTGAGCGGTACATTACTCGAGTAATGTACCGCTCAACGCCCT-3'

Other clones with same target sequence:

(none)