Construct: shRNA TRCN0000243572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177358.4-6023s21c1
DNA Barcode:
TATTGACTTGTGGCAATTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp945 (240041)
Gene Description:
zinc finger protein 945
Transcript:
RefSeq NM_177358.4 (CURRENT)
Match location:
Position 6023 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATTGACTTGTGGCAATTAAC
Hairpin Sequence:
5'-CCGG-TATTGACTTGTGGCAATTAAC-CTCGAG-GTTAATTGCCACAAGTCAATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATTGACTTGTGGCAATTAACCTCGAGGTTAATTGCCACAAGTCAATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATTGACTTGTGGCAATTAACCTCGAGGTTAATTGCCACAAGTCAATA-3'

Other clones with same target sequence:

(none)