Construct: shRNA TRCN0000243709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001031667.1-1573s21c1
DNA Barcode:
ACCCATCCTCACAAGCTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gsk3a (606496)
Gene Description:
glycogen synthase kinase 3 alpha
Transcript:
RefSeq NM_001031667.1 (CURRENT)
Match location:
Position 1573 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCATCCTCACAAGCTTTAA
Hairpin Sequence:
5'-CCGG-ACCCATCCTCACAAGCTTTAA-CTCGAG-TTAAAGCTTGTGAGGATGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCATCCTCACAAGCTTTAACTCGAGTTAAAGCTTGTGAGGATGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCATCCTCACAAGCTTTAACTCGAGTTAAAGCTTGTGAGGATGGGT-3'

Other clones with same target sequence:

(none)