Transcript: Mouse NM_001031667.1

Mus musculus glycogen synthase kinase 3 alpha (Gsk3a), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Gsk3a (606496)
Length:
2276
CDS:
192..1664

Additional Resources:

NCBI RefSeq record:
NM_001031667.1
NBCI Gene record:
Gsk3a (606496)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001031667.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000216595 GAGAGATGAACCCTAACTATA pLKO.1 1225 CDS 100% 13.200 18.480 N Gsk3a n/a
2 TRCN0000219577 TGGGTGTAAATAGATTGTTAT pLKO.1 2033 3UTR 100% 13.200 18.480 N Gsk3a n/a
3 TRCN0000010340 GACTAGAGGGCAGAGGTAAAT pLKO.1 1765 3UTR 100% 13.200 10.560 N GSK3A n/a
4 TRCN0000243710 GACTAGAGGGCAGAGGTAAAT pLKO_005 1765 3UTR 100% 13.200 10.560 N Gsk3a n/a
5 TRCN0000308048 GACTAGAGGGCAGAGGTAAAT pLKO_005 1765 3UTR 100% 13.200 10.560 N GSK3A n/a
6 TRCN0000199832 GCTGGACCACTGCAATATTGT pLKO.1 689 CDS 100% 5.625 4.500 N GSK3A n/a
7 TRCN0000308032 GCTGGACCACTGCAATATTGT pLKO_005 689 CDS 100% 5.625 4.500 N GSK3A n/a
8 TRCN0000243709 ACCCATCCTCACAAGCTTTAA pLKO_005 1573 CDS 100% 13.200 9.240 N Gsk3a n/a
9 TRCN0000243713 CTTGGACAAAGGTGTTCAAAT pLKO_005 1279 CDS 100% 13.200 9.240 N Gsk3a n/a
10 TRCN0000243711 GAACCGAGAACTGCAGATTAT pLKO_005 662 CDS 100% 13.200 9.240 N Gsk3a n/a
11 TRCN0000243712 TAAGCTGGACCACTGCAATAT pLKO_005 686 CDS 100% 13.200 9.240 N Gsk3a n/a
12 TRCN0000200688 GACATCAAAGTGATTGGTAAT pLKO.1 552 CDS 100% 10.800 7.560 N Gsk3a n/a
13 TRCN0000219575 GGCTCATTCGGAGTAGTATAC pLKO.1 573 CDS 100% 10.800 7.560 N Gsk3a n/a
14 TRCN0000010339 CATTCTCATCCCTCCTCACTT pLKO.1 1508 CDS 100% 4.950 3.465 N GSK3A n/a
15 TRCN0000192450 CGAGCTGTATTTGAATCTGGT pLKO.1 752 CDS 100% 2.640 1.848 N Gsk3a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001031667.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000492039 GATCCAATAGAATTTCCAAGTTCG pLX_317 29.5% 89% 93.8% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000488511 ATTGGACCACCCGGTGGCGCATGG pLX_317 21.2% 88.9% 93.6% V5 (many diffs) n/a
3 ccsbBroadEn_14660 pDONR223 94.8% 88.9% 93.2% None (many diffs) n/a
4 TRCN0000479763 TAATGAACCCTGGACGGGTCTAAT pLX_317 23.9% 88.9% 93.2% V5 (many diffs) n/a
5 ccsbBroadEn_14661 pDONR223 100% 88.8% 47.9% None (many diffs) n/a
6 ccsbBroad304_14661 pLX_304 0% 88.8% 47.9% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000469862 TTCATGTATTTTACGTTTCATCGC pLX_317 23.9% 88.8% 47.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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