Construct: shRNA TRCN0000243852
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_133791.4-2762s21c1
- DNA Barcode:
- CAGATCAGCCTGGCGGATTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Wwc2 (52357)
- Gene Description:
- WW, C2 and coiled-coil domain containing 2
- Transcript:
- RefSeq NM_133791.4 (CURRENT)
- Match location:
- Position 2762 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000243852)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAGATCAGCCTGGCGGATTTA
- Hairpin Sequence:
-
5'-CCGG-CAGATCAGCCTGGCGGATTTA-CTCGAG-TAAATCCGCCAGGCTGATCTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGATCAGCCTGGCGGATTTACTCGAGTAAATCCGCCAGGCTGATCTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGATCAGCCTGGCGGATTTACTCGAGTAAATCCGCCAGGCTGATCTG-3'
Other clones with same target sequence:
(none)
