Construct: shRNA TRCN0000243875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130859.2-1300s21c1
DNA Barcode:
GAAGGACTGTGACCTCTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Card10 (105844)
Gene Description:
caspase recruitment domain family, member 10
Transcript:
RefSeq NM_130859.2 (CURRENT)
Match location:
Position 1300 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGACTGTGACCTCTATAA
Hairpin Sequence:
5'-CCGG-GAAGGACTGTGACCTCTATAA-CTCGAG-TTATAGAGGTCACAGTCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGACTGTGACCTCTATAACTCGAGTTATAGAGGTCACAGTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGACTGTGACCTCTATAACTCGAGTTATAGAGGTCACAGTCCTTC-3'

Other clones with same target sequence:

(none)