Construct: shRNA TRCN0000243918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177045.3-2901s21c1
DNA Barcode:
CCGTAACCGCTGCATAGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cc2d1b (319965)
Gene Description:
coiled-coil and C2 domain containing 1B
Transcript:
RefSeq NM_177045.3 (CURRENT)
Match location:
Position 2901 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTAACCGCTGCATAGTAAT
Hairpin Sequence:
5'-CCGG-CCGTAACCGCTGCATAGTAAT-CTCGAG-ATTACTATGCAGCGGTTACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTAACCGCTGCATAGTAATCTCGAGATTACTATGCAGCGGTTACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTAACCGCTGCATAGTAATCTCGAGATTACTATGCAGCGGTTACGG-3'

Other clones with same target sequence:

(none)