Construct: shRNA TRCN0000244487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023284.3-1590s21c1
DNA Barcode:
GCTTCTGTTTGGCATAGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nuf2 (66977)
Gene Description:
NUF2, NDC80 kinetochore complex component
Transcript:
RefSeq NM_023284.3 (CURRENT)
Match location:
Position 1590 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCTGTTTGGCATAGTTAT
Hairpin Sequence:
5'-CCGG-GCTTCTGTTTGGCATAGTTAT-CTCGAG-ATAACTATGCCAAACAGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCTGTTTGGCATAGTTATCTCGAGATAACTATGCCAAACAGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCTGTTTGGCATAGTTATCTCGAGATAACTATGCCAAACAGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000215593