Construct: shRNA TRCN0000215593
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_023284.3-1590s1c1
- DNA Barcode:
- GCTTCTGTTTGGCATAGTTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nuf2 (66977)
- Gene Description:
- NUF2, NDC80 kinetochore complex component
- Transcript:
- RefSeq NM_023284.3 (CURRENT)
- Match location:
- Position 1590 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000215593)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTTCTGTTTGGCATAGTTAT
- Hairpin Sequence:
-
5'-CCGG-GCTTCTGTTTGGCATAGTTAT-CTCGAG-ATAACTATGCCAAACAGAAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTTCTGTTTGGCATAGTTATCTCGAGATAACTATGCCAAACAGAAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTTCTGTTTGGCATAGTTATCTCGAGATAACTATGCCAAACAGAAGC-3'
Other clones with same target sequence:
-
TRCN0000244487
