Construct: shRNA TRCN0000244542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026138.2-2107s21c1
DNA Barcode:
GTGCCAGCTACAGTTCGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Soga3 (67412)
Gene Description:
SOGA family member 3
Transcript:
RefSeq NM_026138.2 (CURRENT)
Match location:
Position 2107 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCCAGCTACAGTTCGTTAA
Hairpin Sequence:
5'-CCGG-GTGCCAGCTACAGTTCGTTAA-CTCGAG-TTAACGAACTGTAGCTGGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCCAGCTACAGTTCGTTAACTCGAGTTAACGAACTGTAGCTGGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCCAGCTACAGTTCGTTAACTCGAGTTAACGAACTGTAGCTGGCAC-3'

Other clones with same target sequence:

(none)