Construct: shRNA TRCN0000245869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039397.2-1002s21c1
DNA Barcode:
ATTCACAGGCATGGGTGTCTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D28 (254272)
Gene Description:
TBC1 domain family member 28
Transcript:
RefSeq NM_001039397.2 (CURRENT)
Match location:
Position 1002 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCACAGGCATGGGTGTCTC
Hairpin Sequence:
5'-CCGG-ATTCACAGGCATGGGTGTCTC-CTCGAG-GAGACACCCATGCCTGTGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCACAGGCATGGGTGTCTCCTCGAGGAGACACCCATGCCTGTGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCACAGGCATGGGTGTCTCCTCGAGGAGACACCCATGCCTGTGAAT-3'

Other clones with same target sequence:

(none)