Construct: shRNA TRCN0000245872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039397.2-1769s21c1
DNA Barcode:
TCACCGTGTTTCATGCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D28 (254272)
Gene Description:
TBC1 domain family member 28
Transcript:
RefSeq NM_001039397.2 (CURRENT)
Match location:
Position 1769 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACCGTGTTTCATGCTATTT
Hairpin Sequence:
5'-CCGG-TCACCGTGTTTCATGCTATTT-CTCGAG-AAATAGCATGAAACACGGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACCGTGTTTCATGCTATTTCTCGAGAAATAGCATGAAACACGGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACCGTGTTTCATGCTATTTCTCGAGAAATAGCATGAAACACGGTGA-3'

Other clones with same target sequence:

(none)