Construct: shRNA TRCN0000246138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001447.2-3317s21c2
DNA Barcode:
TACTACTGGTTGACGGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAT2 (2196)
Gene Description:
FAT atypical cadherin 2
Transcript:
RefSeq NM_001447.2 (CURRENT)
Match location:
Position 3317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTACTGGTTGACGGTATTA
Hairpin Sequence:
5'-CCGG-TACTACTGGTTGACGGTATTA-CTCGAG-TAATACCGTCAACCAGTAGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTACTGGTTGACGGTATTACTCGAGTAATACCGTCAACCAGTAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTACTGGTTGACGGTATTACTCGAGTAATACCGTCAACCAGTAGTA-3'

Other clones with same target sequence:

(none)