Construct: shRNA TRCN0000246390

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029376.2-584s21c1
DNA Barcode:
GAGTTGAACCTGAGTTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Speer4a (75657)
Gene Description:
spermatogenesis associated glutamate (E)-rich protein 4A
Transcript:
RefSeq NM_029376.2 (CURRENT)
Match location:
Position 584 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246390)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTGAACCTGAGTTGTAAA
Hairpin Sequence:
5'-CCGG-GAGTTGAACCTGAGTTGTAAA-CTCGAG-TTTACAACTCAGGTTCAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTGAACCTGAGTTGTAAACTCGAGTTTACAACTCAGGTTCAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTGAACCTGAGTTGTAAACTCGAGTTTACAACTCAGGTTCAACTC-3'

Other clones with same target sequence:

  1. TRCN0000191643