Construct: shRNA TRCN0000246407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148932.2-795s21c1
DNA Barcode:
CCTCCAGACAGCAAGCTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pom121 (107939)
Gene Description:
nuclear pore membrane protein 121
Transcript:
RefSeq NM_148932.2 (CURRENT)
Match location:
Position 795 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAGACAGCAAGCTATTC
Hairpin Sequence:
5'-CCGG-CCTCCAGACAGCAAGCTATTC-CTCGAG-GAATAGCTTGCTGTCTGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAGACAGCAAGCTATTCCTCGAGGAATAGCTTGCTGTCTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAGACAGCAAGCTATTCCTCGAGGAATAGCTTGCTGTCTGGAGG-3'

Other clones with same target sequence:

(none)