Construct: shRNA TRCN0000246447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178720.4-631s21c1
DNA Barcode:
CCGTTGGAATACCTGGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zpld1 (239852)
Gene Description:
zona pellucida like domain containing 1
Transcript:
RefSeq NM_178720.4 (CURRENT)
Match location:
Position 631 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTGGAATACCTGGTTAAT
Hairpin Sequence:
5'-CCGG-CCGTTGGAATACCTGGTTAAT-CTCGAG-ATTAACCAGGTATTCCAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTGGAATACCTGGTTAATCTCGAGATTAACCAGGTATTCCAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTGGAATACCTGGTTAATCTCGAGATTAACCAGGTATTCCAACGG-3'

Other clones with same target sequence:

(none)