Construct: shRNA TRCN0000246504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175207.4-1168s21c1
DNA Barcode:
GTAGGTCCAGCTCGATGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd9 (74251)
Gene Description:
ankyrin repeat domain 9
Transcript:
RefSeq NM_175207.4 (CURRENT)
Match location:
Position 1168 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGGTCCAGCTCGATGTGAA
Hairpin Sequence:
5'-CCGG-GTAGGTCCAGCTCGATGTGAA-CTCGAG-TTCACATCGAGCTGGACCTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGGTCCAGCTCGATGTGAACTCGAGTTCACATCGAGCTGGACCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGGTCCAGCTCGATGTGAACTCGAGTTCACATCGAGCTGGACCTAC-3'

Other clones with same target sequence:

(none)