Construct: shRNA TRCN0000246589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010750.3-1002s21c1
DNA Barcode:
CCTTTAAATGCACCGGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mab21l1 (17116)
Gene Description:
mab-21-like 1 (C. elegans)
Transcript:
RefSeq NM_010750.3 (CURRENT)
Match location:
Position 1002 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTAAATGCACCGGGATTT
Hairpin Sequence:
5'-CCGG-CCTTTAAATGCACCGGGATTT-CTCGAG-AAATCCCGGTGCATTTAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTAAATGCACCGGGATTTCTCGAGAAATCCCGGTGCATTTAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTAAATGCACCGGGATTTCTCGAGAAATCCCGGTGCATTTAAAGG-3'

Other clones with same target sequence:

(none)