Construct: shRNA TRCN0000246593
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010750.3-1347s21c1
- DNA Barcode:
- AGTCGGACTGGGACGAATCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mab21l1 (17116)
- Gene Description:
- mab-21-like 1 (C. elegans)
- Transcript:
- RefSeq NM_010750.3 (CURRENT)
- Match location:
- Position 1347 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000246593)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGTCGGACTGGGACGAATCTT
- Hairpin Sequence:
-
5'-CCGG-AGTCGGACTGGGACGAATCTT-CTCGAG-AAGATTCGTCCCAGTCCGACT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGTCGGACTGGGACGAATCTTCTCGAGAAGATTCGTCCCAGTCCGACTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGTCGGACTGGGACGAATCTTCTCGAGAAGATTCGTCCCAGTCCGACT-3'
Other clones with same target sequence:
(none)
