Construct: shRNA TRCN0000246748

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025496.1-500s21c1
DNA Barcode:
AGAACCTGCACAACTCCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdrt4 (66338)
Gene Description:
CMT1A duplicated region transcript 4
Transcript:
RefSeq NM_025496.1 (CURRENT)
Match location:
Position 500 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246748)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACCTGCACAACTCCAAAT
Hairpin Sequence:
5'-CCGG-AGAACCTGCACAACTCCAAAT-CTCGAG-ATTTGGAGTTGTGCAGGTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACCTGCACAACTCCAAATCTCGAGATTTGGAGTTGTGCAGGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACCTGCACAACTCCAAATCTCGAGATTTGGAGTTGTGCAGGTTCT-3'

Other clones with same target sequence:

(none)