Construct: shRNA TRCN0000246775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177184.3-9535s21c1
DNA Barcode:
CCCTATCCGGTGCCCTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps13c (320528)
Gene Description:
vacuolar protein sorting 13C
Transcript:
RefSeq NM_177184.3 (CURRENT)
Match location:
Position 9535 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATCCGGTGCCCTATTAA
Hairpin Sequence:
5'-CCGG-CCCTATCCGGTGCCCTATTAA-CTCGAG-TTAATAGGGCACCGGATAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATCCGGTGCCCTATTAACTCGAGTTAATAGGGCACCGGATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATCCGGTGCCCTATTAACTCGAGTTAATAGGGCACCGGATAGGG-3'

Other clones with same target sequence:

(none)