Construct: shRNA TRCN0000246778
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_030137.2-89s21c1
- DNA Barcode:
- TAACGGAGGCTCACAAGGAAG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Cstad (78617)
- Gene Description:
- CSA-conditional, T cell activation-dependent protein
- Transcript:
- RefSeq NM_030137.2 (CURRENT)
- Match location:
- Position 89 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000246778)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TAACGGAGGCTCACAAGGAAG
- Hairpin Sequence:
-
5'-CCGG-TAACGGAGGCTCACAAGGAAG-CTCGAG-CTTCCTTGTGAGCCTCCGTTA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTAACGGAGGCTCACAAGGAAGCTCGAGCTTCCTTGTGAGCCTCCGTTATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATAACGGAGGCTCACAAGGAAGCTCGAGCTTCCTTGTGAGCCTCCGTTA-3'
Other clones with same target sequence:
(none)
