Construct: shRNA TRCN0000247194

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028306.3-748s21c1
DNA Barcode:
ATCTGGAAACAACCGGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hspa12b (72630)
Gene Description:
heat shock protein 12B
Transcript:
RefSeq NM_028306.3 (CURRENT)
Match location:
Position 748 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247194)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTGGAAACAACCGGCTAAA
Hairpin Sequence:
5'-CCGG-ATCTGGAAACAACCGGCTAAA-CTCGAG-TTTAGCCGGTTGTTTCCAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTGGAAACAACCGGCTAAACTCGAGTTTAGCCGGTTGTTTCCAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTGGAAACAACCGGCTAAACTCGAGTTTAGCCGGTTGTTTCCAGAT-3'

Other clones with same target sequence:

(none)