Transcript: Mouse NM_028306.3

Mus musculus heat shock protein 12B (Hspa12b), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Hspa12b (72630)
Length:
2945
CDS:
121..2178

Additional Resources:

NCBI RefSeq record:
NM_028306.3
NBCI Gene record:
Hspa12b (72630)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_028306.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000217728 GGTTAGAGTTGGCTAACTTTG pLKO.1 2501 3UTR 100% 1.080 1.512 N Hspa12b n/a
2 TRCN0000247196 ACCTACAGGAAAGCTATAAAT pLKO_005 2702 3UTR 100% 15.000 10.500 N Hspa12b n/a
3 TRCN0000247197 GCTCTACTTTGAGAAGTTTAA pLKO_005 531 CDS 100% 13.200 9.240 N Hspa12b n/a
4 TRCN0000247195 AGCAGCTTGGGTGGATCTAAC pLKO_005 1263 CDS 100% 10.800 7.560 N Hspa12b n/a
5 TRCN0000247194 ATCTGGAAACAACCGGCTAAA pLKO_005 748 CDS 100% 10.800 7.560 N Hspa12b n/a
6 TRCN0000200943 CAGAAGGCATCTTTCACAGTT pLKO.1 452 CDS 100% 4.950 3.465 N Hspa12b n/a
7 TRCN0000202256 GAGCGAGTGTATGCTTGAGAA pLKO.1 693 CDS 100% 4.950 3.465 N Hspa12b n/a
8 TRCN0000201893 CATCTGGAAACAACCGGCTAA pLKO.1 747 CDS 100% 4.050 2.835 N Hspa12b n/a
9 TRCN0000130271 CCATCGACTTTCTTTCCAACT pLKO.1 2156 CDS 100% 4.050 2.835 N HSPA12B n/a
10 TRCN0000247198 AGAGAGCAGAGCGAGTGTATG pLKO_005 685 CDS 100% 10.800 6.480 N Hspa12b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_028306.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09435 pDONR223 100% 85.7% 93.8% None (many diffs) n/a
2 ccsbBroad304_09435 pLX_304 0% 85.7% 93.8% V5 (many diffs) n/a
3 TRCN0000479290 TATCCTCAAGCTTACCCGAACAGT pLX_317 21.2% 85.7% 93.8% V5 (many diffs) n/a
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