Construct: shRNA TRCN0000247196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028306.3-2702s21c1
DNA Barcode:
ACCTACAGGAAAGCTATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hspa12b (72630)
Gene Description:
heat shock protein 12B
Transcript:
RefSeq NM_028306.3 (CURRENT)
Match location:
Position 2702 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTACAGGAAAGCTATAAAT
Hairpin Sequence:
5'-CCGG-ACCTACAGGAAAGCTATAAAT-CTCGAG-ATTTATAGCTTTCCTGTAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTACAGGAAAGCTATAAATCTCGAGATTTATAGCTTTCCTGTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTACAGGAAAGCTATAAATCTCGAGATTTATAGCTTTCCTGTAGGT-3'

Other clones with same target sequence:

(none)